Milano was speaking at Politicon in Los Angeles on a panel with several other #MeToo figures when Laura Loomer, a former Project Veritas operative and conservative freelance journalist, confronted her.
She asked Milano to publicly condemn Sarsour, saying: 'I want to ask you right now to disavow Linda Sarsour because she is a supporter of Sharia law and under Sharia law, women are oppressed, forced to wear a hijab.'
Loomer was escorted out of the room as she tried to finish her questions while Milano and others yelled back at her in defense of Sarsour: 'No she doesn't.'
Alyss Milano, pictured at Politicon on Sunday, when she was confronted over her friendship with a controversial Muslim women's rights activist.
Milano was questioned over her friendship with Linda Sarsour (left). The pair are pictured at a January protest calling for asylum for illegal immigrants. Sarsour has been accused of promoting Sharia law and antisemitism in the past
As Loomer was led out of the room to the jeers of the crowd, Milano said: 'If we don't know how to talk to one another we'll never evolve.'
When Loomer argued back, 'I asked you a polite question!', the actress said that she had applied a 'very distinct tone'.
She waved Loomer off saying: 'Thank you for your question. God bless you.'
Sarsour has been accused in the past of encouraging Muslims to wage jihad against President Trump.
Earlier this year, she was labeled anti-Semitic by Courtney Love and was accused of inventing stories to drum up support for her community by supporting a Somali woman who made a refuted claim that she was attacked by a group of white men in a violent hate crime.
Journalist Laura Loomer (shown at the microphone in a floral top) asked Milano (center in the row of speakers) to disavow Sarsour at Politicon on Sunday.
Loomer was thrown out of the room before being able to finish speaking. The crowd jeered at her and Milano criticized her 'very distinct tone'.
Sarsour's alleged anti-antisemitism is linked to her support of Louis Farrakhan, leader of Nation of Islam whose repeatedly offensive remarks about Jews have been universally condemned, and her position on the Palestinian-Israeli conflict.
Laura Loomer, the conservative freelance journalist who confronted Milano.
Sarsour is a Palestinian-American and leader of the Muslim grassroots movement MPower Change.
In previous speeches, she has called on Muslim Americans to support the Palestinian American cause and said they were 'complicit in murder' if they did not.
'If you’re on the side of the oppressor, or you’re defending the oppressor, or you’re actually trying to humanize the oppressor, then that’s a problem sisters and brothers, and we got to be able to say: that is not the position of the Muslim American community,' she said at the Islamic Society of North America (ISNA) Convention in Houston in September.
She has compared 'right wing Zionists' and Blue Lives Matters campaigners to neo-Nazis and said that she would 'not be silenced' by them.
Linda Sarsour is one of the organizers of the Women's March and is a Palestinian-American activist. She is pictured in September at a protest against the appointment of the then Supreme Court nominee Brett Kavanaugh.
Sarsour has made controversial remarks in the past including supporting Saudi Arabia, which is prolifically behind on women's rights, by saying it puts the US to 'shame' with its maternity leave.
Sarsour has also been criticized for supporting Sharia law. Above is a 2015 tweet.
In 2015, she tweeted: 'You'll know when you're living under Sharia Law if suddenly all your loans and credit cards become interest free. Sounds nice, doesn't it?'
She has also been accused of supporting rock throwing for once posting a picture of a Palestinian child facing a row of police with rocks in his hands, writing: 'The definition of courage'.
Sarsour did not respond to DailyMail.com's inquiries on Monday morning when asked to make her position on Sharia law clear.
In previous speeches, she has insisted she is not antisemitic and said she is committed to ironing out all levels of racial prejudice including towards Jews.
The depths of her friendship with Milano are unclear.
The pair are united in their positions on migration and women's rights and have posed together at marches for each cause in the past.